MECHANISMS OF PHOTOSENSITIZED DNA CLEAVAGE

Photosensitization may promote DNA damages such as nucleic acid oxidat ion or single strand breaks via three main pathways: hydroxyl radicals attack, electron transfer process or oxidation by singlet oxygen. Whi le direct production of OH. by photosensitization is rarely observed, the mechanism of DNA attack by OH. is now well established on the basi s of informations provided by water radiolysis experiments. Some dyes may also induce single strand breaks via an electron transfer occurrin g from a nucleobase to the sensitizer in the excited state. This proce ss generates base radical cations identical to those arising from DNA photoionisation. These radicals may undergo deprotonation or dehydrati on to form the same neutral radicals as those produced by OH. but with a slightly different pattern. In contrast, while many sensitizers pro duce singlet oxygen, the mechanism of DNA damages induced by this way is still unclear. In this case the guanine moiety in nucleosides or in DNA is selectively altered leading to the formation of 8 oxoG or 8 ox odG and FapyGua. The mechanism of single strand breaks formation by si nglet oxygen is discussed in this overview.