Mj. Blackledge et al., CONFORMATIONAL BACKBONE DYNAMICS OF THE CYCLIC DECAPEPTIDE ANTAMANIDE- APPLICATION OF A NEW MULTICONFORMATIONAL SEARCH ALGORITHM-BASED ON NMR DATA, Biochemistry, 32(41), 1993, pp. 10960-10974
A general procedure for the analysis of biomolecular structures by NMR
in the presence of rapid conformational dynamics has been applied to
the study of the cyclic decapeptide antamanide. Two-dimensional experi
ments, relaxation measurements in the rotating frame, and homo- and he
teronuclear coupling constant determinations have been used to charact
erize the dynamic properties of the molecule, in combination with a no
vel search algorithm for investigating multiconformational equilibria.
Direct evidence for the presence of a conformational exchange process
with an activation energy of approximately 20 kJ mol-1 and an exchang
e lifetime of approximately 25 mus at 320 K has been obtained from rot
ating frame relaxation measurements. This evidence is combined with th
e information derived from the multiconformational search algorithm ME
DUSA to propose sets of structures that coexist in a dynamic exchange
equilibrium.