THE INFLUENCE OF 4-DEGREES-C STORAGE ON PROLIFERATIVE POTENTIAL OF HUMAN BONE-MARROW CD34- TRANSPLANTOLOGICAL IMPLICATIONS( CELLS )

The possibility of storage of human bone marrow CD34+ cells at 4-degre es-C was investigated for bone marrow transplantational purposes. The cells were placed in Iscove medium supplemented with 20% serum (15% bo vine calf serum 5% human AB serum) for three weeks at 4-degrees-C. Dur ing the storage time, clonogenecity of granulocyto-monocytic (CFU-GM) erythropoietic (BFU-E) and megakaryocytic (CFU-Meg) progenitors was in vestigated. It was found that it is possible to keep human CD34+ cells at 4-degrees-C, at least for a few days before transplantation. At da y four of the storage, the number of CFU-GM and BFU-E cells still exce eded 50% of the cells present at day 0. However, we have found that CF U-Meg in comparison to other progenitors are much more sensitive to me tabolic storage stress. This enhanced sensitivity of megakaryocytic pr ogenitors could explain, at least partially, the well known phenomenon of retardation of the thrombopoietic recovery in patients undergoing bone marrow transplantations.