THE NUCLEOTIDE-SEQUENCE OF A 39 KB SEGMENT OF YEAST CHROMOSOME-IV - 12 NEW OPEN READING FRAMES, 9 KNOWN GENES AND ONE GENE FOR GLY-TRANSFER-RNA

The complete nucleotide sequence of a 39 090 bp segment from the left arm of yeast chromosome IV was determined. Twenty-one open reading fra mes (ORFs) longer than 100 amino acids and a Gly-tRNA gene were discov ered. Nine of the 21 ORFs (D0892, D1022, D1037, D1045, D1057, D1204, D 1209, D1214, D1219) correspond to the previously sequenced Saccharomyc es cerevisiae genes for the NAD-dependent glutamate dehydrogenase (GDH ), the secretory component (SHR3), the GABA transport protein (UGA4), the high mobility group-like protein (NHP2), the hydroxymethylbilane s ynthase (HEM3), the methylated DNA protein-cysteine S-methyltransferas e (MGT1), a putative sugar transport protein, the Shm1 protein (SHIM1) and the anti-silencing protein (ASF2). The inferred amino acid sequen ces of 11 ORFs show significant similarity with known proteins from va rious organisms, whereas the remaining ORF does not share any similari ty with known proteins. The nucleotide sequence has been entered in th e EMBL data Library under the Accession Number X99000.