THE SEQUENCE OF 32 KB ON THE LEFT ARM OF YEAST CHROMOSOME-XII REVEALS6 KNOWN GENES, A NEW MEMBER OF THE SERIPAUPERINS FAMILY AND A NEW ABCTRANSPORTER HOMOLOGOUS TO THE HUMAN MULTIDRUG-RESISTANCE PROTEIN

The analysis of a 32 kb DNA fragment from cosmid 2G12 on the left arm of chromosome XII identifies 14 open reading frames (ORFs) numbered L0 948 to L1325, a new tRNA for proline, a delta remnant and two putative ARS. Six ORFs have been previously identified: HSP104, SSA2, SPA2, KN S1, DPS1/APS and SDC25. Three putative ORFs have significant homology with known proteins: L0968 is a new member of the very large 'seripaup erins' family, comprising at least 20 yeast members; L1313 is a new AB C transporter highly homologous to the yeast cadmium resistance protei n Ycf1p and to the human multidrug resistance protein hMRP1; the C-ter minal part of L1325 present in our sequence is very homologous to the fruit fly abdominal segment formation protein Pumilio. Finally, two OR Fs, L1201 and L1205, have weak homology with two yeast hypothetical pr oteins of unknown function identified by the yeast systematic sequenci ng genome. Since our nucleotide sequence overlaps by 11.6 kb the cosmi d 2B18 sequenced by Miosga and Zimmerman (1996) on the right end, we h ave not reported here the analysis of the ORFs L1313, L1321 and L1325. The complete nucleotide sequence of 32,088 bp and the deduced ORFs we re submitted to the EMBL database under Accession Number X97560.