EFFECTS OF 4 DIFFERENT EXTENDERS AND 3 DIFFERENT FREEZING RATES ON POSTTHAW VIABILITY OF DOG SEMEN

Ejaculates were collected from 17 dogs. After initial evaluation the e jaculates were split into four equal aliquots and diluted with either Triladyl, Pipes, IMV Universal or Tris-fructose-citric acid extender, all containing egg yolk and glycerol. The extended semen was frozen in 0.5 ml straws at a slow, intermediate or fast freezing rate. After th awing, the percentage of progressively motile spermatozoa and the velo city of forward motion were evaluated. There was no difference between the extenders with regard to progressive motility. The slow, intermed iate and fast freezing rates resulted in the highest, intermediate and lowest motility values, respectively, regardless of extender. All fou r extenders were equally suitable for freezing canine semen at a slow freezing rate.