IDENTIFICATION OF 108-K, 93-K, AND 42-K GLYCOPROTEINS OF BOVINE HERPESVIRUS-1 BY MONOCLONAL-ANTIBODIES

Three glycoproteins of bovine herpesvirus-1 (BHV-1) other than glycopr oteins g I, g III, and g IV were identified by monoclonal antibody (MA b) analyses. Monoclonal antibodies were obtained by immunization of mi ce with either BHV-1 envelope or virus infected cells, from which the glycoproteins g I, gIII, and gIV were removed by immunoaffinity. In th e latter immunization procedure mice were tolerized either against nor mal cellular antigens with or without glycoproteins g I, g III, g IV, and nucleocapsid. From 154 anti-BHV-1 hybridomas isolated, 39 MAbs pre cipitated a 108 K glycoprotein. Two other glycoproteins of respectivel y 42 K and 93 K were precipitated each by one MAb. These three glycopr oteins were detected in infected cell lysate. Nine anti-108 K glycopro tein MAbs neutralized BHV-I infectivity and three non-neutralizing MAb s were able to reduce plaque development when virus was grown in the p resence of these MAbs. It is therefore suggested that this glycoprotei n is involved in viral entry into the cell and in cell-to-cell spread of the virus.