Mamj. Vanzandvoort et al., SPECTROSCOPIC STUDY OF CHLOROPHYLL-A IN ORGANIC-SOLVENTS AND POLYMERIZED ANHYDROUS POLYVINYL MATRIX, Photochemistry and photobiology, 58(4), 1993, pp. 600-606
In this paper we present a spectroscopic study of chlorophyll a in sol
utions and in anhydrous polyvinyl alcohol films. Absorption, excitatio
n and emission spectra, combined with fluorescence lifetime and time-r
esolved anisotropy measurements show that chlorophyll a in anhydrous p
olyvinyl alcohol films exists in a purely monomeric state. Furthermore
, it appears that the monomeric chlorophyll a exhibits an efficient ex
citation energy transfer in this polyvinyl alcohol matrix. These resul
ts are rationalized in terms of a model in which the chlorophyll a mol
ecules are located within pockets, formed by the polymer chains. It is
concluded that the chlorophyll a-anhydrous polyvinyl alcohol film is
a suitable system for studying energy transfer processes, especially b
ecause the factors governing energy transfer such as mutual orientatio
n and separation of the molecules can easily be controlled.