CHARACTERIZATION OF A PC12 CELL SUB-CLONE (PC12-C41) WITH ENHANCED NEURITE OUTGROWTH CAPACITY - IMPLICATIONS FOR A MODULATORY ROLE OF HIGH-MOLECULAR-WEIGHT TAU IN NEURITOGENESIS

To address the means by which diversity of neuronal morphology is gene rated, we have isolated and characterized naturally occurring variants of rat PC12 pheochromocytoma cells that exhibit altered neurite outgr owth properties in response to nerve growth factor (NGF). We describe here a PC12 cell sub-clone, designated PC12-clone 41 (PC12-C41), that displays significant increases in neurite abundance and stability when compared with the parental line. This difference does not appear to b e due to an altered sensitivity or responsiveness to NGF or to a more rapid rate of neurite extension. Because of the role of the cytoskelet on in neuritogenesis, we examined a panel of the major cytoskeletal pr oteins (MAP 1.2/1B, beta-tubulin, chartins, peripherin, and high and l ow molecular weight (HMW and LMW) taus) whose levels and/or extent of phosphorylation are regulated by NGF in PC12 cultures. Although most c ytoskeletal proteins showed little difference between PC12 and PC12-C4 1 cells (+/- NGF treatment), there was a significant contrast between the two lines with respect to tau expression. In particular, while NGF increases the total specific levels of tau in both cell types to simi lar extents (by about twofold), the proportion comprising HMW tau is t hreefold higher in the PC12-C41 clone than in PC12 cells. A comparable difference was observed under substratum conditions that were nonperm issive for neurite outgrowth and so this effect was not merely a conse quence of the differential neuritogenic capacities of the two lines. T he distinction between the expression of HMW and LMW taus in PC12 and PC12-C41 cells (+/- NGF) was also observed at the level of the message s encoding these proteins. Such findings indicate that initiation of n eurite outgrowth in PC12 cultures does not require a massive induction of tau expression and raise the possibility that HMW and LMW taus may have differential capacities for modulating neuronal morphology.