SUBSTRATE-SPECIFICITY AND STEREOSELECTIVITY OF HYDROLYTIC ENZYMES FROM BREVIBACTERIUM-IMPERIALE B222

Authors
BIANCHI D BATTISTEL E CESTI P GOLINI P TASSINARI R
Citation
D. Bianchi et al., SUBSTRATE-SPECIFICITY AND STEREOSELECTIVITY OF HYDROLYTIC ENZYMES FROM BREVIBACTERIUM-IMPERIALE B222, Applied microbiology and biotechnology, 40(1), 1993, pp. 53-56
Citations number
8
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Applied microbiology and biotechnology → ACNP
ISSN journal
01757598
Volume
40
Issue
1
Year of publication
1993
Pages
53 - 56
Database
ISI
SICI code
0175-7598(1993)40:1<53:SASOHE>2.0.ZU;2-U
Abstract
Four different hydrolytic enzymes were isolated and partially purified from Brevibacterium imperiale B222 cells. The stereoselectivity of ea ch enzyme was assayed by using the nitrile, amide and esters derivativ es of 2-aryloxypropionic acid. Within the cellular pool of hydrolytic enzymes, a non-stereoselective nitrile hydratase, a stereoselective am idase and two partially stereoselective esterases of opposite enantiom eric preference were found.