P. Sun et al., CHIRAL SEPARATIONS USING DEXTRAN AND BOVINE SERUM-ALBUMIN AS RUN BUFFER ADDITIVES IN AFFINITY CAPILLARY ELECTROPHORESIS, Journal of chromatography, 648(2), 1993, pp. 475-480
A method utilizing dextran as a run buffer additive in addition to bov
ine serum albumin (BSA) for chiral separation by means of affinity cap
illary electrophoresis (ACE) has been developed. By adding different a
mounts of dextran to the run buffer, the net velocity of BSA can be ad
justed to a desired rate. Enantiomers of some drugs [ibuprofen (IB) an
d leucovorin (LV)] and amino acids (dansyl-DL-leucine and dansyl-DL-no
rvaline) were separated on a capillary with 20 cm effective length by
this method. The effect of dextran concentration on the retention of B
SA and resolution of sample enantiomers was studied. Enantiomers of ma
ndelic acid (MA), which have very weak affinity for BSA, were also res
olved. Qualitative information pertaining to the binding interaction o
f the samples with BSA can be obtained by this method.