M. Kokko, GAS-CHROMATOGRAPHIC SCREENING FOR NEOSTIGMINE AND PHYSOSTIGMINE USINGTEMPERATURE-PROGRAMMED RETENTION INDEXES, Journal of chromatography, 648(2), 1993, pp. 501-506
Temperature-programmed gas chromatographic retention indices, relative
to n-alkane (C standard) and n-alkylbis(trifluoromethyl)phosphine sul
phide (M standard) homologous series, were determined for two low-vola
tile, highly toxic carbamates. neostigmine and physostigmine on SE-54
fused-silica capillary columns. The influence of temperature programme
and sample concentration on the absolute values of the indices were e
valuated. Reproducibility of the indices was good in both linear and m
ultistep temperature programmes, but the reproducibility and peak shap
es were better in the multistep temperature programme. When the compou
nds were present in high concentration, C standards and a flame ioniza
tion detector were used successfully, but multidetector M standards, w
hich also can be seen with the selective alkali thermoionization detec
tor, were superior when concentrations were low. Similarly M standards
which can be seen also with other selective detectors (electron-captu
re, flame photometric and photoionization) could be a suitable index s
tandard series for screening of different types of compounds containin
g heteroatoms.