SIMPLE REPETITIVE DNA-SEQUENCES IN THE SOYBEAN (GLYCINE-MAX) GENOME

We attempted to identify motifs of simple repetitive sequences in the soybean genome by Southern hybridization with digoxigenin-labeled olig onucleotide probes and chemiluminescent detection methods. Signals wer e detected with all the fourteen probes used. The organization and abu ndance of the tested sequence motifs varied considerably. Among the pr obes investigated, (CT)8, (GAA)5 and (GTG)5 produced strong smear sign als over a wide range of molecular weights. These sequence motifs coul d be used to isolate informative locus-specific markers for linkage ma pping. The clearest banding patterns were obtained by using (AAT)6 as a probe, and this may be applied to fingerprinting and genome scanning .