IMAGE-PROCESSING OF ELECTRON-MICROGRAPHS FOR LIGHT-SCATTERING CALCULATIONS

Theoretical light scattering calculations, when applied to models base d on structures seen in electron micrographs of corneal stroma, requir e knowledge of the constituent fibril positions and radii. Obtaining t his information manually is a difficult and time-consuming task. In or der to facilitate this problem, we present a simple and flexible compu ter algorithm that allows the process to be automated using a Macintos h computer. The accuracy of the results is checked by comparing light scattering calculations using fibril positions and radii found manuall y with those found by the computer. The results show that the computer algorithm is a viable and accurate means of obtaining the needed data .