LIPID-PEROXIDATION IN ETHYLENE-GLYCOL INDUCED HYPEROXALURIA AND CALCIUM-OXALATE NEPHROLITHIASIS

Purpose: To determine if lipid peroxidation plays a role in renal inju ry associated with experimental nephrolithiasis. Materials and Methods : Hyperoxaluria was produced in rats by ethylene glycol in drinking wa ter. At 15, 30 and 60 days of treatment, urinary oxalate, lipid peroxi de, calcium oxalate crystals, enzymes and tissue lipid peroxide were m easured. Results: Urinary oxalate increased significantly at all time periods and was associated with crystalluria. Lipid peroxides in kidne y tissue and urine increased at all time periods. Tissue calcium oxala te crystal deposit; from 0 to 1+ were present on day 15, but present i n all animals on days 30 and 60. Renal tubular cell damage was confirm ed by an increase in urinary marker enzymes. Conclusions: Renal cell d amage is associated with lipid peroxide production indicating cell inj ury due to the production of free radicals. The damage appears due pri marily to hyperoxaluria and is augmented by crystal deposition in the renal tubules.