EVALUATION OF INJURY PRESERVATION IN PIG-KIDNEY COLD-STORAGE BY PROTON NUCLEAR-MAGNETIC-RESONANCE SPECTROSCOPY OF URINE

The isolated perfused kidney (IPK) model from pig was used to assess i nitial renal functions after cold flush and a 24- and 48-hour cold sto rage preservation (CSP) with Euro-Collins (EC) and to determine ischem ia reperfusion (IR) impairment by biochemical, histological and proton nuclear magnetic resonance (NMR) spectroscopy of urine analysis. Eigh teen pig kidneys were used. There were three experimental groups: Grou p 1-control kidneys flushed with cold heparinized saline, Group 2-cold flush followed by 24-hour CSP and reperfusion, Group 3-cold flush fol lowed by 48-hour CSP and reperfusion. Kidneys were perfused for 2-hour at 37.5C for the determination of exogenous creatinine clearance (CCr ), perfusion flow rate (PFR) and tubular function. Functional paramete rs and histological data are very poor after prolonged cold ischemia. Levels of tubular injury markers determined by proton NMR spectroscopy analysis were significantly higher with a longer ischemic time correl ating well with the increased degree of renal functional and anatomic impairment. A new peak detected only in the 48-hour ischemic group was identified. In this model, proton NMR spectroscopy may be a useful te chnique in the evaluation of both new preservation solutions or method s.