S. Mathur et al., IDENTIFICATION OF AN INTRACELLULAR PYRIMIDINE-SPECIFIC ENDORIBONUCLEASE FROM BACILLUS-SUBTILIS, Journal of bacteriology, 175(20), 1993, pp. 6717-6720
Two intracellular RNases which were easily separated by fractionation
on strong anion- or cation-exchange resins were identified from Bacill
us subtilis. One cleaved any phosphodiester bond, while the second cle
aved only pyrimidine-N bonds. The enzyme with pyrimidine-N specificity
was approximately 15 kDa, had a pH optimum of approximately 6.2, degr
aded C-C bonds approximately 10 times faster than U-U bonds, and was c
ompletely inactive against single-stranded DNA. The enzyme is called R
Nase C and may be the first reported broad-specificity endoribonucleas
e from B. subtilis.