IDENTIFICATION OF AN INTRACELLULAR PYRIMIDINE-SPECIFIC ENDORIBONUCLEASE FROM BACILLUS-SUBTILIS

Citation
S. Mathur et al., IDENTIFICATION OF AN INTRACELLULAR PYRIMIDINE-SPECIFIC ENDORIBONUCLEASE FROM BACILLUS-SUBTILIS, Journal of bacteriology, 175(20), 1993, pp. 6717-6720
Citations number
29
Categorie Soggetti
Microbiology
Journal title
ISSN journal
00219193
Volume
175
Issue
20
Year of publication
1993
Pages
6717 - 6720
Database
ISI
SICI code
0021-9193(1993)175:20<6717:IOAIPE>2.0.ZU;2-Y
Abstract
Two intracellular RNases which were easily separated by fractionation on strong anion- or cation-exchange resins were identified from Bacill us subtilis. One cleaved any phosphodiester bond, while the second cle aved only pyrimidine-N bonds. The enzyme with pyrimidine-N specificity was approximately 15 kDa, had a pH optimum of approximately 6.2, degr aded C-C bonds approximately 10 times faster than U-U bonds, and was c ompletely inactive against single-stranded DNA. The enzyme is called R Nase C and may be the first reported broad-specificity endoribonucleas e from B. subtilis.