A. Montero et al., EFFECT OF OUABAIN AND HYPOTHALAMIC, HYPOPHYSARY INHIBITORY FACTOR ON RAT MESANGIAL CELL-PROLIFERATION, Journal of cardiovascular pharmacology, 22, 1993, pp. 190000035-190000037
The mitogenic effect of a low-molecular-weight, Na+,K+-ATPase inhibito
ry factor isolated from bovine hypothalamus and hypophysis (hypothalam
ic hypophysary inhibitory factor, HHIF) and ouabain on cultured rat me
sangial cells was examined. Ouabain induced a potent stimulation of th
e [H-3]thymidine incorporation to DNA, ranging from threefold for a do
se of 10(-7) M to seven times for 10(-5) M. The amount of proteins per
well also increased in a dose-dependent way, which was already signif
icant from basal values for the lower dose used. HHIF also induced a d
ose-dependent stimulation of [H-3]thymidine incorporation into DNA, ra
nging from 1.7 times for a concentration of 0.02 U/ml to 4.5 times for
the concentration of 2 U/ml. The amount of protein per well also incr
eased with HHIF, but the increase was significant only for the higher
dose used. These data allow us to conclude that HHIF could play a role
in the vascular hypertrophy that is associated with the pathogenesis
of hypertension.