DETECTION OF ENZYMATIC POLYMORPHISM IN 5 SESAME VARIETIES (SESAMUM-INDICUM L) USING ISOELECTRIC-FOCUSING AND DISC GEL-ELECTROPHORESIS

In order to identify varieties through isozyme using isoelectric focus ing and/or disc gel electrophoresis, a study to detect polymorphism in five varieties of sesame (Sesamum indicum L.) for acid phosphatase, e sterase D, esterase A, esterase B, malate dehidrogenase, peroxidase an d isocitrate lyase was carried out. Protein extract from seeds or leav es of 7, 14 and 21 days after emergency were used. Phenotypic variatio n was found only for seed extract. There were polymorphisms in all enz ymes. Two phenotypes were found for peroxidase, three for acid phospha tase and esterase A, four for isocitrate lyase . For malate dehidrogen ase, esterase D and esterase B, each variety showed a particular pheno type.