We have previously reported that in Guatemala, the calcium, iron, and
zinc contents of tortillas from rural areas are higher than that of to
rtillas from urban centers.. This study examines variation in the calc
ium, iron, zinc and copper content of tortilla according to the implem
ents used for making tortillas and inquires as to whether preparation
effects mediate rural-urban variation in tortilla mineral content. Tor
tilla samples and information on how the tortillas were prepared were
collected from the female heads of a total of 50 households from three
rural, two semi-urban and one low income urban community. Samples of
lime used for making tortillas were collected from 31 households. To g
rind masa, a hand mill was found to be used in some rural households w
hereas a motorized mill predominated in the semi-urban and urban areas
. Most women used grinding stones called the <<mano y metate>> to furt
her refine the texture of the masa. Tortillas prepared with the combin
ed use of the hand mill and <<mano y metate>> had a significantly (p<0
.05) higher iron content. Use of the <<mano y metate>> was also associ
ated with a significantly (p<0.05) higher zinc content. These results
suggest that the use of certain grinding implements may mediate rural-
urban variation in tortilla iron and zinc content. The cooking surface
, pot used for nixtamalization, source of water, and amount of lime us
ed did not significantly account for variation in the content of these
minerals.