THE USE OF BIOLUMINESCENCE TO STUDY THE BEHAVIOR OF LISTERIA-MONOCYTOGENES WHEN ATTACHED TO SURFACES

Authors
WALKER AJ HOLAH JT DENYER SP STEWART GSAB
Citation
Aj. Walker et al., THE USE OF BIOLUMINESCENCE TO STUDY THE BEHAVIOR OF LISTERIA-MONOCYTOGENES WHEN ATTACHED TO SURFACES, Colloids and surfaces. A, Physicochemical and engineering aspects, 77(3), 1993, pp. 225-229
Citations number
21
Categorie Soggetti
Chemistry Physical
Journal title
Colloids and surfaces. A, Physicochemical and engineering aspects → ACNP
ISSN journal
09277757
Volume
77
Issue
3
Year of publication
1993
Pages
225 - 229
Database
ISI
SICI code
0927-7757(1993)77:3<225:TUOBTS>2.0.ZU;2-U
Abstract
A recombinant derivative of Listeria monocytogenes engineered to expre ss the luxAB genes of Vibrio harveyi has a bioluminescent phenotype th at provides a rapid monitor of microbial viability. This phenotype was employed to follow bacterial attachment on to stainless steel surface s, to monitor the growth of microcolonies upon these surfaces and to a ssess the resistance of the attached cells following biocide challenge . Real-time bioluminescence measurements provided data equivalent to v iable count determinations, and the organism was found to become more resistant to the biocide phenol upon attachment to stainless steel sur faces.