EFFECTS OF NORDIHYDROGUAIARETIC ACID ON THE CHEMILUMINESCENCE OF MURINE PHAGOCYTES

Antioxidative effects of nordihydroguaiaretic acid (NDGA) on murine bo ne marrow phagocytes were studied using luminol- and lucigenin-amplifi ed chemiluminescence (CL). NDGA applied in vitro strongly suppressed t he opsonized zymosan particles stimulated CL response in a dose-depend ent manner (in concentrations of 0.3-30 mug NDGA/ml), thus confirming its antioxidant activity. However, no effects were observed in mice an d their serum samples when investigated one minute to one hour after a n i.p. administration of NDGA. These differences suggest that NDGA may undergo a rapid metabolism in vivo.