P1 AND COSMID CLONES DEFINE THE ORGANIZATION OF 280 KB OF THE MOUSE H-2-COMPLEX CONTAINING THE CPS-1 AND HSP70 LOCI

A 280 kilobase (kb) contig was isolated from mouse genomic P1 and cosm id libraries, using as probes human cDNA and genomic DNA fragments tha t map in the interval between the second component of complement and t umor necrosis factor genes of the HLA complex. The clone contig demons trates synteny of eleven mouse genes that are homologous to genes init ially mapped within the human major histocompatibility complex. These include the mouse homologs of BAT2 (HLA-B-associated transcript 2) thr ough BAT9 and also three HSP70-related genes. Five P1 clones form a co ntig of 240 kb that spans from BAT9 through BAT3. Twelve cosmid clones are arranged in three contigs that confirm most of the structure of t he P1 contig and link the mouse BAT3 homolog to the BAT2 homolog appro ximately 15 kb farther telomeric. Polymorphic DNA markers within the c loned region were used to map the cleft palate susceptibility-l (Cps-l ) locus to the interval between Hsp70.1 and BAT6 (valyl-tRNA synthetas e). This refines the location of the Cps-1 locus to a 45 kb region con tained in the H2-124 P1 insert.