COMPARATIVE-STUDY OF THE INTERACTIONS OF CISPLATIN AND CARBOPLATIN WITH NUCLEOTIDES USING UV RESONANCE RAMAN-SPECTROSCOPY

We have obtained the UV excited resonance Raman spectra of five mononu cleotides bound to cisplatin and to carboplatin using excitation in re sonance with the first electronic absorption bands of the nucleotide b ases. Substantial changes in the spectra are observed. following inter action with both platinum drugs, indicating modifications to nucleotid e structure. Pt (II) binds to base portions of the nucleotide molecule s, altering their normal modes of vibration significantly. We present comparative data of cisplatin and carboplatin, and discuss the implica tions of these results. The kinetics of the drug/nucleotide reactions differ, but final products are found to be similar. (C) 1993 John Wile y & Sons, Inc.