ESTERASE-30 (ES-30) OF THE HOUSE MOUSE - BIOCHEMICAL-CHARACTERIZATIONAND GENETICS OF A NEW CARBOXYLESTERASE ISOZYME LINKED TO CLUSTER-2 LOCI ON CHROMOSOME 8
A. Ronai et al., ESTERASE-30 (ES-30) OF THE HOUSE MOUSE - BIOCHEMICAL-CHARACTERIZATIONAND GENETICS OF A NEW CARBOXYLESTERASE ISOZYME LINKED TO CLUSTER-2 LOCI ON CHROMOSOME 8, Biochemical genetics, 31(7-8), 1993, pp. 279-294
A new carboxylesterase isozyme (EC 3.1.1.1), designated ES-30, is desc
ribed in mouse liver. Two phenotypes were distinguished, ES-30A, a pos
sible null type, was found in SPE/Pas and in other lines derived from
Mus spretus, and ES-30B was found in BALB/cJ and other laboratory inbr
ed strains. ES-30B is characterized by a distinct electrophoretic band
when stained using 5-bromoindoxyl acetate as the substrate. After iso
lation and purification from other esterases by ion-exchange chromatog
raphy and molecular sieving, the molecular mass was estimated by two i
ndependent methods to be 62 and 64 kDa, respectively. The activity of
ES-30B is higher in adult males than in females and can be stimulated
in vivo by testosterone. The distribution of phenotypes on the progeny
of a backcross series suggests a separate locus, Es-30, with the alle
le a for absence and b for presence of the isozyme. Locus Es-30 is sho
wn to be closely linked to Es-2 and to Es-7 of cluster-2 on chromosome
8. The gene order Es-9-Got-2-(Es-2, Es-7, Es-30) is suggested.