BINDING-STUDIES WITH RECOMBINANT HUMAN SERUM-ALBUMIN OBTAINED BY EXPRESSION OF A SYNTHETIC GENE IN YEAST - STEREOSELECTIVE BINDING AND ALLOSTERIC INTERACTION WITH BENZODIAZEPINE AND COUMARIN LIGANDS

The specific ligand binding ability of recombinant human serum albumin produced in yeast using the synthetic gene was studied by affinity ch romatographic method. It was found that synthetic protein possesses th ose stereoselective binding and binding interactions for several chira l benzodiazepine and coumarin compounds which are characteristic of th e natural human serum albumin, suggesting identical tertiary structure s.