E. Manor et al., TNF AND PGE(2) IN HUMAN MONOCYTE-DERIVED MACROPHAGES INFECTED WITH CHLAMYDIA-TRACHOMATIS, Mediators of inflammation, 2(5), 1993, pp. 367-371
IN this study levels of prostaglandin E(2) (PGE(2)), tumour necrosis f
actor (TNF) and interleukin-1 (IL-1) alpha in medium from monocyte der
ived macrophages (MdM) infected with Chlamydia trachomatis (L(2)/434/B
u or R biovars). TNF and PGE(2) were found in both cases while IL-1 al
pha was not detected. Both TNF and PGE(2) levels were higher in the me
dium of the MdM infected with R biovars. TNF reached maximum levels 24
h postinfection, and then declined, while PGE(2) levels increased con
tinuously during the infection time up to 96 h post-infection. Additio
n of dexamethasone inhibited production of TNF and PGE(2). Inhibition
of PGE(2) production by indomethacin resulted in increased production
of TNF, while addition of PGE(2) caused partial inhibition of TNF prod
uction from infected MdM.