THE GROWTH OF MOUSE HYBRIDOMA CELLS BETWEEN PEYERS PATCH LYMPHOCYTE AND MYELOMA CELL IN THE GASTROINTESTINAL-TRACT - A MODEL FOR HUMAN MULTIPLE LYMPHOMATOUS POLYPOSIS OF THE GASTROINTESTINAL-TRACT

To elucidate the mechanism of multiple lesions in human primary lympho ma or multiple lymphomatous polyposis of the gastrointestinal tract, w e developed a experimental model utilizing mouse hybridoma cells. Syng eneic mice were immunized with human hemoglobin via gastric intubation and a hybridoma clone (13c) between the Peyer's patch cell and mouse myeloma cell was obtained. The 13c cells were infused into normal syng eneic mice through a lateral tail vein. About 2 months after injection mice were sacrificed and their viscera were scrutinized histologicall y. All 11 mice receiving 13c showed multiple tumors at the colon, micr oscopic massive invasions at the small intestine, and scattered invasi ons at Peyer's patch, mesenteric lymph nodes and the spleen, exhibitin g the similar distribution patterns as in human multiple lymphomatous polyposis of the gastrointestinal tract. ME-1 or MU-1 cells (hybridoma s between P3 and mouse spleen cell) were distributed at the liver, spl een and paraaortic lymph nodes. Such migration patterns of 13c cells a nd their adherence to vessel walls suggest that multiple invasions may be lymphocyte homing receptor-mediated phenomena.