POLYMORPHONUCLEAR NEUTROPHILS ADHERENCE, SUPEROXIDE ANION (O-2(-)) PRODUCTION AND H(B)A(1) LEVEL IN DIABETIC-PATIENTS

It was established that metabolic disturbances accompanying the impair ment of diabetes control induced polymorphonuclear neutrophil (PMN) ac tivation. Therefore, we evaluated the influence of diabetes control, m easured as HbA(1), level on superoxide anions production and neutrophi ls adherence to foreign surface. Twenty patients with insulin-dependen t or non-insulin-dependent diabetes being in different state of metabo lic control were studied. It was revealed that PMN from diabetics demo nstrated significantly increased unstimulated superoxide anion product ion (11.07 +/- 1.25 vs. 4.15 +/- 1.24 nmol O-2(-)/10(6) PMN/10 min). S timulated O-2(-) production did not reach the same levels as controls (21.80 +/- 1.34 vs. 23.67 +/- 4.36 nmol O-2(-)/10(6) PMN/10 min). Ther e were highly significant correlations between unstimulated O-2(-) pro duction and HbA(1), levels (r=0.86). Adherence of diabetic PMN suspend ed in autologous plasma was significantly increased in comparison to c ontrols (12.76 +/- 1.20 vs. 6.04 +/- 1.99 cells/mm(2)). However, these data did not correlate with HbA(1) level (r=0.06). In conclusion our results seems to indicate that metabolic control of diabetes influence PMN function.