The protease nexins are protease inhibitors which regulate key blood c
oagulation proteases and which appear to be involved in certain physio
logical and pathological processes in the brain. Protease nexin-1 (PN-
1), a potent inhibitor of thrombin, can regulate processes on cultured
neurons and astrocytes. Protease nexin-2 (PN-2), a potent inhibitor o
f coagulation factor XIa, is identical to the secreted form of the Alz
heimer's amyloid beta-protein precursor. In the present studies, PN-1
and PN-2 were analyzed in different tissues of monkey using monoclonal
antibodies for either quantitative immunoblotting or specific [I-125]
protease-binding assays. PN-1 was detected only in brain. PN-2 was mos
t abundant in brain, followed by testis and to a lesser extent kidney.
Other tissues examined including spinal cord, heart, pancreas, spleen
, liver, lung and muscle were essentially devoid of both PN-1 and PN-2
. Within the brain, the levels of PN-1 and PN-2 were highest in the pa
rietal cortex and lowest in the cerebellum and brainstem. The thalamus
and striatum contained intermediate amounts of both proteins. Aged Ce
bus monkey cerebral cortical tissue contained slightly lower levels of
PN-1 than did the middle-aged or young monkey tissue. The co-distribu
tion of PN-1 and PN-2 in brain, their relative abundance in brain cort
'', and previous studies on their functions suggest that in the brain
they may participate in the regulation of blood coagulation and cell g
rowth and differentiation.