CLONING AND CHARACTERIZATION OF THE RAT 5-HT(5B)RECEPTOR - EVIDENCE THAT THE 5-HT(5B)RECEPTOR COUPLES TO A G-PROTEIN IN MAMMALIAN-CELL MEMBRANES

Authors
WISDEN W PARKER EM MAHLE CD GRISEL DA NOWAK HP YOCCA FD FELDER CC SEEBURG PH VOIGT MM
Citation
W. Wisden et al., CLONING AND CHARACTERIZATION OF THE RAT 5-HT(5B)RECEPTOR - EVIDENCE THAT THE 5-HT(5B)RECEPTOR COUPLES TO A G-PROTEIN IN MAMMALIAN-CELL MEMBRANES, FEBS letters, 333(1-2), 1993, pp. 25-31
Citations number
11
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
333
Issue
1-2
Year of publication
1993
Pages
25 - 31
Database
ISI
SICI code
0014-5793(1993)333:1-2<25:CACOTR>2.0.ZU;2-7
Abstract
A gene encoding a novel G protein-coupled 5-hydroxytryptamine (5-HT) r eceptor, termed 5-HT5B, was cloned. The ligand binding profile of this receptor is distinct from that of other cloned 5-HT receptors. The 5- HT5B receptor couples to a G protein in COS1 cell membranes; however, activation of the 5-HT5B receptor does not appear to alter either cAMP accumulation or phosphoinositide turnover in a variety of fibroblast cell lines. In the rat brain, 5-HT5B gene expression occurs predominan tly in the medial habenulae and hippocampal CA1 cells of the adult. Li ttle expression is seen during embryonic development.