TRIMERIC MUTANT BACTERIORHODOPSIN, D85N, SHOWS A MONOPHASIC CD SPECTRUM

Citation
M. Kataoka et al., TRIMERIC MUTANT BACTERIORHODOPSIN, D85N, SHOWS A MONOPHASIC CD SPECTRUM, FEBS letters, 333(1-2), 1993, pp. 111-113
Citations number
26
Categorie Soggetti
Biophysics,Biology
Journal title
ISSN journal
00145793
Volume
333
Issue
1-2
Year of publication
1993
Pages
111 - 113
Database
ISI
SICI code
0014-5793(1993)333:1-2<111:TMBDSA>2.0.ZU;2-A
Abstract
The structure of mutant bacteriorhodopsin (bR), D85N, was examined by CD and X-ray diffraction at pH 7. The absorption maximum of D85N at pH 7 is located at 605 nm, which is similar to the acid-blue form of wil d-type bR. D85N shows a monophasic CD band, the maximum of which is at 575 nm, although the crystalline arrangement and the trimeric structu re is maintained. The acid-blue form of wild-type bR shows a biphasic CD despite the similarity in absorption spectra.