THE PRO SEQUENCE OF LACTASE-PHLORHIZIN HYDROLASE IS REQUIRED FOR THE ENZYME TO REACH THE PLASMA-MEMBRANE - AN INTRAMOLECULAR CHAPERONE

Authors
OBERHOLZER T MANTEI N SEMENZA G
Citation
T. Oberholzer et al., THE PRO SEQUENCE OF LACTASE-PHLORHIZIN HYDROLASE IS REQUIRED FOR THE ENZYME TO REACH THE PLASMA-MEMBRANE - AN INTRAMOLECULAR CHAPERONE, FEBS letters, 333(1-2), 1993, pp. 127-131
Citations number
25
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
333
Issue
1-2
Year of publication
1993
Pages
127 - 131
Database
ISI
SICI code
0014-5793(1993)333:1-2<127:TPSOLH>2.0.ZU;2-2
Abstract
Various cDNAs coding for part or all of human pre-pro lactase-phlorizi n hydrolase (pre-proLPH) were transfected into COS cells and the subce llular location of the lactase-related proteins assessed. Only the com plete proLPH reached the plasma membrane. LPH without the pro sequence , and a construct containing the pro sequence and the lactase domain o f mature LPH, accumulated intracellularly; the pro sequence with no ma ture domain was secreted. We conclude that the pro sequence is importa nt for LPH to be transported to the cell surface.