C. Perennes et al., PETUNIA P34(CDC2) PROTEIN-KINASE ACTIVITY IN G(2)M CELLS OBTAINED WITH A REVERSIBLE CELL-CYCLE INHIBITOR, MIMOSINE, FEBS letters, 333(1-2), 1993, pp. 141-145
Protoplasts isolated from petunia leaf mesophyll are non-cycling cells
mostly with 2C content. Cells regenerating from protoplast culture en
ter mitosis after 48 h. This experimental model is used to relate p34c
dc2 kinase activity to cell cycle phase. Our results show that the his
tone H1 phosphorylation, and hence p34cdc2 kinase activity, peaks with
G2+early M cell cycle phase. However, a trace kinase activity was alr
eady present when most cells were entering S phase. To obtain a maximu
m of cells in G1+S phases, the protoplast culture was treated with the
rare amino acid, mimosine. Mimosine blocked plant cells derived from
protoplast culture both at G1 and in early and mid S phase. Despite th
e increased G1+S level, p34cdc2 kinase activity did not increase. This
suggests that the trace activity appearing when the majority of cells
are entering S does not correspond to any putative p34cdc2 activation
at G1/S transition but to the activation of the minor 4C population i
nitially present in the leaf: the hypothesis remains that p34cdc2 kina
se activity is solely related to G2+M phase in petunia.