IMMUNOCHEMICAL AND P-32 POSTLABELING METHODS IN BENZO[A]PYRENE DNA-ADDUCTS DETERMINATION

A wide literature exists on DNA-adducts determination since their form ation represents a crucial step in carcinogenesis. However, it is to b e underlined that in most studies P-32-postlabelling methods, endowed with greater sensibility, have been utilized. Instead, in the present study Authors focused their attention on immunochemical determination of DNA adducts which does not require radiolabelled materials; moreove r, it can ben exploited for its capacity of discriminating among diffe rent types of adducts. Benzo[a]pyrene has been selected for its widesp read diffusion in the environment and the results of the two methods h ave been compared.