Energy-dependent quenching of chlorophyll fluorescence and its reversi
on in intact isolated spinach chloroplasts were recorded at temperatur
es between 0 and 20-degrees-C. A temperature-dependence of the quenchi
ng formation (and of quenching relaxation) corresponding to Q10 values
between 2 and 3 was found. Long-term cold acclimation of the plants r
esulted in a decrease of the fluorescence decline half-time. The final
steady-state extent of quenching (q(E)) was not influenced by tempera
ture shifts or cold acclimation.