ENHANCED SELECTIVITY IN FLOW-INJECTION ANALYSIS FOR L-AMINO-ACIDS USING ELECTRODIALYSIS WITH AMINO-ACID OXIDATION

A method has been developed for continuous determination of the concen tration of one amino acid in a mixture, using electrodialysis combined with a flow-injection system. Using a non-specific L-amino acid oxida se column and electrochemical detection of enzymatically produced hydr ogen peroxide, the concentration of a particular amino acid in a mixtu re could be determined by first performing a separation step using an electrodialysis unit. Using the separation of arginine as a model syst em, it was possible to determine the concentration of this amino acid in the presence of two or three of a group of nine amino acids.