GENOTYPIC TYPING AND PHYLOGENETIC ANALYSIS OF SALMONELLA-PARATYPHI B AND S-JAVA WITH IS200

Salmonella paratyphi B and Salmonella java are biovars of common serot ype 1.4.[51.12: b: 1.2 which respectively cause human paratyphoid feve r and gastroenteritis. In order to define genotypes and phylogenetic r elationships in this group, we examined representative strains for res triction fragment length polymorphisms (RFLPs) in and around the 16S r ibosomal RNA (rrn) genes, and the five to eleven insertion sites of th e Salmonella-specific DNA insertion sequence IS200. One of four 16S rr n profiles was predominant. and was shared by the majority of strains. irrespective of their designation as S. paratyphi B or S. java. On th e other hand, thirteen unique IS200 profiles were found and this techn ique was able to distinguish, for the first time, distinct genotypes f or S. paratyphi B and S. java. One of the S. paratyphi B profiles, Spj -IP1.0, represented a globally-distributed clone. Greater diversity wa s detected within IS200 profiles of S. java than within those of S. pa ratyphi B. IS200 profiles described a phylogenetic complex in which st rains of both biovars could be placed. They constituted reproducible m olecular fingerprints, which could be compared in a band-matching data base suitable for molecular epidemiological typing.