J. Stanley et al., HELICOBACTER-CANIS SP-NOV, A NEW SPECIES FROM DOGS - AN INTEGRATED STUDY OF PHENOTYPE AND GENOTYPE, Journal of General Microbiology, 139, 1993, pp. 2495-2504
A group of Campylobacter-like organisms (CLOs) were isolated from the
faeces of diarrhoeic or healthy dogs, constituting 4% of all CLOs from
this source. Since they formed a unique DNA homology group within the
genus Helicobacter, and exhibited distinctive phenotypic properties,
they were collectively termed the HC group. A polyphasic taxonomic ana
lysis was made of this group. The phenotype of four dog isolates and a
single human isolate was unique and could be distinguished bacteriolo
gically from other helicobacters. Electron microscopic ultrastructure
revealed defining characteristics of Helicobacter. The 16S rRNA gene o
f the nominated type strain NCTC 12739T was sequenced, and its analysi
s delineated the group as a new species of Helicobacter. This conclusi
on was supported by relative DNA homology and whole-cell protein elect
rophoretic patterns. We therefore propose the name Helicobacter canis
sp. nov. for this group. The species most closely related to H. canis
sp. nov. were H. cinaedi, 'Flexispira rappini' and H. fennelliae. A sp
ecies-specific recombinant DNA probe was cloned from NCTC 12739T for u
se in routine laboratory identification and epidemiological studies. T
he faecal source, bile tolerance and lack of urease activity of H. can
is sp. nov. suggest that this new Helicobacter species colonizes the l
ower bowel rather than the stomach.