M. Musil et J. Gallo, DETERMINATION OF BROAD BEAN STAIN VIRUS SEROTYPES BY ENZYME-LINKED-IMMUNOSORBENT-ASSAY, Acta virologica, 37(4), 1993, pp. 265-270
Enzyme-linked immunosorbent assay (ELISA) was used to determine the de
gree of serological specificity of two serotypes of broad bean stain v
irus (BBSV) and their relationship to red clover mottle virus (RCMV).
Optimal conditions for the differentiation by ELISA of the two viruses
and the two BBSV serotypes were established. BBSV isolates from Vicia
sativa belonged to serotype I, those from pea plants (F1, Kow 60) to
serotype II, and isolates from Lens culinaris differed from these two
serotypes. ELISA revealed no antigenic differences between 22 RCMV iso
lates which showed the same degree of serological relationship to both
BBSV serotypes.