The feasibility of using bulk segregant analysis to identify molecular
markers for disease resistance genes in oats was investigated, utiliz
ing random primers in conjunction with polymerase chain reaction techn
ology. Random primers were screened for the amplification of polymorph
ic DNA fragments on two pools of genomic DNA isolated from plants that
were homozygous for the presence and absence of the crown rust resist
ance gene Pc68. Ten primers were identified that amplified polymorphic
DNA fragments. Of these,one was tightly linked, in repulsion, to the
target gene, while the other nine were not linked to this trait. The r
elatively low cost of polymerase chain reaction technology, coupled wi
th rapid leaf disc genomic DNA extraction techniques should result in
the effective use of this linked marker in oat breeding selection prog
rams.