HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF NITROFURANTOIN IN PLASMA

A reversed-phase high-performance liquid chromatographic method for th e analysis of nitrofurantoin in rabbit plasma was developed. Acetanili de was used as the internal standard. The chromatography was performed using a C-18 column; the mobile phase consisted of 75:25 water to met hanol; a flow rate of 0.9 ml/min; and UV detection at 270 nm. Retentio n times were 5.8 and 10.1 min for nitrofurantoin and acetanilide, resp ectively. Recovery of nitrofurantoin added to plasma in the concentrat ion range of 0.05-5 mug/ml was found to be greater than 92%. This proc edure was used for the analysis of plasma samples collected over time following the oral administration of nitrofurantoin tablets to rabbits .