Tc. Hsu et al., CYTOGENETIC STUDIES ON THE IN-VITRO GENOTOXICITY OF 4-NITROQUINOLINE-1-OXIDE ON HUMAN-LYMPHOCYTES, International journal of oncology, 3(5), 1993, pp. 823-826
Using the UV-mimetic mutagen 4-nitroquinoline-1-oxide (4NQO) to induce
genetic damage in human cells (lymphoblastoid lines and primary cultu
res of peripheral blood samples), chromosome aberrations were induced
by treating the cells with 4NQO at 1 X 10(-5) M for 24 h. The overwhel
ming majority of chromosome aberrations was of the chromatid (S + G2)
type instead of the chromosome (G1) type. The most common chromatid ab
errations were simple breaks, isochromatid breaks, and chromatid excha
nges. When the number of chromatid breaks per cell value was used as a
measurement for 4NQO sensitivity, lymphoblastoid cells from a xeroder
ma pigmentosum patient showed the highest sensitivity, followed by the
cells of two melanoma patients and normal persons. These preliminary
results suggest that 4NQO may be employed to develop an assay system a
s a biomarker for determining UV sensitivity in the human population.