STEP-FORTIFICATIONS OF NUTRIENTS IN MAMMALIAN-CELL CULTURE

A series of high-density media for mammalian cell culture were develop ed by step-fortifications of most nutrient components in RPMI-1640 med ium. Each medium constituting the series was constructed to meet in vi tro cell growth limitations. Four different cell lines were cultivated in the media series, and their growth characteristics were observed. Maximum cell densities varied in the range of 0.4 to 1.3 x 10(7) cells /mL, depending on cell lines. Cell growth responses to each of the med ia series were analyzed in terms of cell density and cell mass. Step i ncreases of cell mass in the range of 1.3 to 3.7 g/L were observed acc ording to the step-fortifications of nutrients. Also, the characterist ics of each cell line were compared in terms of metabolic yields and s pecific productions of lactic acid and ammonium ion. The effect of ste p-fortifications of nutrients on the production of monoclonal antibody was also examined. Apparent differences in metabolic characteristics among cell lines were observed. Experimental results suggested that th e different cell sizes and metabolic characteristics of each cell line resulted in cell-line-specific responses to the step-fortifications. The significant influence of nutritional fortifications on high-densit y culture of mammalian cells was evaluated. (C) 1993 John Wiley & Sons , Inc.