EXPRESSION OF A RETROPOSON-LIKE SEQUENCE UPSTREAM OF THE PUTATIVE TRYPANOSOMA-BRUCEI VARIANT SURFACE GLYCOPROTEIN GENE-EXPRESSION SITE PROMOTER

Citation
Mj. Lodes et al., EXPRESSION OF A RETROPOSON-LIKE SEQUENCE UPSTREAM OF THE PUTATIVE TRYPANOSOMA-BRUCEI VARIANT SURFACE GLYCOPROTEIN GENE-EXPRESSION SITE PROMOTER, Molecular and cellular biology, 13(11), 1993, pp. 7036-7044
Citations number
45
Categorie Soggetti
Biology
ISSN journal
02707306
Volume
13
Issue
11
Year of publication
1993
Pages
7036 - 7044
Database
ISI
SICI code
0270-7306(1993)13:11<7036:EOARSU>2.0.ZU;2-3
Abstract
We have cloned the region spanning the putative promoter from two vari ant surface glycoprotein gene expression sites that are at each end of chromosome M4 of Trypanosoma brucei IsTat 7. Both expression sites co ntain a retroposon-like sequence (ESR) pseudogene whose 3' end is appr oximately 30 bp upstream of the putative expression site promoter. The ESRs from both expression sites share considerable sequence homology and are related to LINE-like elements, especially the T. brucei ingi r etroposon. Other ESRs are located on large, but not intermediate or mi ni-, chromosomes in the IsTaR 1 serodeme, and the total copy number is 10 to 20, similar to that estimated for variant surface glycoprotein expression sites. No DNA rearrangements in the vicinity of the ESR and putative expression site promoter were detected following antigenic s witches in the IsTaR 1 serodeme. ESR transcripts are present in bloods tream, but not procyclic, forms. Variation in transcript size and sequ ence between bloodstream variant antigenic types implies that only the ESR from the active expression site is transcribed. This pattern of e xpression reflects that of sequences downstream of the putative expres sion site promoter, suggesting that the region of coordinately control led expression extends upstream of this promoter.