Me. Munk et al., ONCHOCERCA-VOLVULUS PROVIDES LIGANDS FOR THE STIMULATION OF HUMAN-GAMMA DELTA T-LYMPHOCYTES EXPRESSING V-DELTA-1 CHAINS/, The Journal of infectious diseases, 168(5), 1993, pp. 1241-1247
Peripheral blood mononuclear cells (PBMC) from 8 onchocerciasis patien
ts, treated or not with ivermectin, were analyzed for phenotypic cell
surface markers. A significant increase (P < .05) in gamma/delta T cel
ls expressing the Vdelta1 chain compared with normal and endemic contr
ols was detected in all patients. PBMC populations from onchocerciasis
patients were not expanded after restimulation with Onchocerca volvul
us antigens in vitro, but both Vdelta1 and Vdelta2 T cells from normal
donors were increased significantly in response to O. volvulus and My
cobacterium tuberculosis (P < .05), respectively. Frozen sections of a
ll 5 onchocerca nodules tested demonstrated an increased number of CD3
+ cells in the vicinity of the adult worm, in all cases expressing the
alpha/beta T cell receptor and in 2 patients also expressing the gamm
a/delta T cell receptor; 60% of T cells expressed the activation marke
r Ki67. These data suggest that O. volvulus provides ligands to Vdelta
1 T cells.