INTERSPECIFIC HYBRID VERIFICATION OF ROSA WITH ISOZYMES

The hybrid origin of 23 rose (Rosa spp.) accessions was examined with three isozymes: acid phosphatase (E.C.3.1.3.2), malate dehydrogenase ( E.C.1.1.1.37), and phosphoglucose isomerase (E.C.5.3.1.9). All three i sozymes were useful for interspecific hybrid verification. This proced ure was effective if the putative parents were known and differed in i sozyme phenotype, To verify the origin of hybrid species or cultivars with hybrid origins, isozymes were useful but limited by the number of generations since the original hybridization and the number of access ions of the putative parental species assayed.