MALIGNANT TRANSFORMATION OF CUTANEOUS LESIONS IN RENAL-ALLOGRAFT PATIENTS - A ROLE FOR HUMAN PAPILLOMAVIRUS

Renal allograft recipients are at greatly increased risk of developing squamous cell carcinomas. As these frequently arise adjacent to areas of multiple viral warts, we have investigated a possible role for hum an papillomavirus in malignant transformation within this population. We established, as primary cultures, keratinocytes from 24 lesions of varying degrees of squamous atypia from 9 patients. Ten of 14 cultures screened for the presence of episomal human papillomavirus DNA were p ositive using a mixed probe for cutaneous human papillomaviruses, alth ough episomal copy was universally lost with continued passage. Three cultures, 2 of which were derived from malignant tissue and 1 from a b enign lesion, were positive when screened with a probe for potentially oncogenic human papillomavirus DNAs 5 or 8. Both positive cultures of malignant origin exhibited extended lifespan and have been briefly ch aracterized by morphology and growth requirements.