CHEMILUMINESCENCE DETERMINATION OF TIOPRONIN AND ITS METABOLITE 2-MERCAPTOPROPIONIC ACID IN HUMAN URINE BY HPLC COUPLED WITH FLOW-INJECTION

Authors
ZHAO YN BAEYENS WRG ZHANG XR CALOKERINOS AC NAKASHIMA K VANDERWEKEN G VANOVERBEKE A
Citation
Yn. Zhao et al., CHEMILUMINESCENCE DETERMINATION OF TIOPRONIN AND ITS METABOLITE 2-MERCAPTOPROPIONIC ACID IN HUMAN URINE BY HPLC COUPLED WITH FLOW-INJECTION, Chromatographia, 44(1-2), 1997, pp. 31-36
Citations number
19
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Chromatographia → ACNP
ISSN journal
00095893
Volume
44
Issue
1-2
Year of publication
1997
Pages
31 - 36
Database
ISI
SICI code
0009-5893(1997)44:1-2<31:CDOTAI>2.0.ZU;2-Y
Abstract
In previous pharmacokinetic studies tiopronin, a drug used for effecti ve treatment of cystinuria and rheumatoid arthritis, and its metabolit e 2-mercaptopropionic acid were analysed by conventional liquid chroma tography with pre- and post-column derivatization and UV detection. No w a novel HPLC-coupled chemiluminescence-flow-injection analysis (CL-F IA) method has been developed for the determination of tiopronin and 2 -mercaptopropionic acid in urine. The method is based on chemiluminesc ence from a Ce(IV) oxidation system sensitized by quinine, as proposed earlier by this group, and flow-injection analysis. The method, which has the advantages of high sensitivity and selectivity, simple sample treatment and prompt production of results, has also been preliminari ly adapted for pharmacokinetic study of tiopronin in urine.