REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF MONOVINYL AND DIVINYL CHLOROPHYLL FORMS USING PYRIDINE-CONTAINING MOBILE PHASES AND A POLYMERIC OCTADECYLSILICA COLUMN
Jl. Garrido et M. Zapata, REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF MONOVINYL AND DIVINYL CHLOROPHYLL FORMS USING PYRIDINE-CONTAINING MOBILE PHASES AND A POLYMERIC OCTADECYLSILICA COLUMN, Chromatographia, 44(1-2), 1997, pp. 43-49
Citations number
37
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
The separation of chlorophyll forms was studied employing a wide bore
polymeric octadecylsilica column and pyridine containing mobile phases
, giving consideration to considering the influence of mobile phase co
mposition and column temperature on the resolution of monovinyl forms
from their divinyl analogues. A method involving gradient elution and
operating at 15 degrees C is proposed for the separation of several po
lar and non-polar mono- and divinyl chlorophylls from etiolated tissue
s of higher plants and from marine phytoplankton. The advantages of py
ridine as a mobile phase additive in the reversed-phase liquid chromat
ography of chlorophylls are discussed.