REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF MONOVINYL AND DIVINYL CHLOROPHYLL FORMS USING PYRIDINE-CONTAINING MOBILE PHASES AND A POLYMERIC OCTADECYLSILICA COLUMN

Citation
Jl. Garrido et M. Zapata, REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF MONOVINYL AND DIVINYL CHLOROPHYLL FORMS USING PYRIDINE-CONTAINING MOBILE PHASES AND A POLYMERIC OCTADECYLSILICA COLUMN, Chromatographia, 44(1-2), 1997, pp. 43-49
Citations number
37
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
ISSN journal
00095893
Volume
44
Issue
1-2
Year of publication
1997
Pages
43 - 49
Database
ISI
SICI code
0009-5893(1997)44:1-2<43:RHLSOM>2.0.ZU;2-J
Abstract
The separation of chlorophyll forms was studied employing a wide bore polymeric octadecylsilica column and pyridine containing mobile phases , giving consideration to considering the influence of mobile phase co mposition and column temperature on the resolution of monovinyl forms from their divinyl analogues. A method involving gradient elution and operating at 15 degrees C is proposed for the separation of several po lar and non-polar mono- and divinyl chlorophylls from etiolated tissue s of higher plants and from marine phytoplankton. The advantages of py ridine as a mobile phase additive in the reversed-phase liquid chromat ography of chlorophylls are discussed.